APIS DPYD qPCR Kit
The APIS DPYD qPCR Kit is designed for fast and efficient detection of all seven Tier 1 DPYD alleles recommended for testing by AMP PGx Working Group (1). Each reaction identifies both wildtype (WT) and variant alleles, directly from blood or extracted genomic DNA (gDNA).
The kit’s rapid turnaround time, cost-effectiveness, and easy integration into existing qPCR workflows, makes it a perfect solution for both centralised and decentralised laboratories.
Analytical Sensitivity
The analytical sensitivity of the kit was evaluated by testing homozygous (wildtype) and heterozygous samples and defined as the minimum amount of DNA necessary to correctly genotype 95% of the samples. The APIS DPYD qPCR Kit can detect seven key DPYD variants in as little as 0.11ng gDNA.
Table 1. DPYD variants and analytical sensitivity of the APIS DPYD qPCR Kit
APIS DPYD qPCR Kit shows excellent concordance with alternative genotyping methods
gDNA extracted from 29 specimens with known DPYD variant status (characterised via NGS, microarrays or qPCR) and one synthetic DNA sample were assessed with the APIS DPYD qPCR Kit.
Table 2. Concordance of the APIS DPYD qPCR Kit with other methods
APIS DPYD qPCR Kit Specifications
The kit has undergone testing as part of APIS unique RUO assay development pipeline, including both in silico and wet laboratory testing. As a result, researchers can depend on the kit for consistent and high-quality detection of DPYD variants. A positive and negative control (PC and NTC), which monitor assay set up and assay performance, are also supplied with the kit.
Ordering Information
| Assay Name | Test Type | Kit Size | Catalogue Number | Price |
|---|---|---|---|---|
| APIS DPYD qPCR Kit | RUO | 24 samples plus controls | 03501 | Available upon request |
To order the APIS DPYD qPCR Kit or to learn more about how our assay can elevate your research capabilities, please contact us below.
1 AMP DPYD Genotyping Recommendations
